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New Zealand Journal of Forestry Science
Fig. 6 | New Zealand Journal of Forestry Science

Fig. 6

From: The occurrence of progesterone 5β-reductase is not limited to the angiosperms: a functional gene was identified in Picea sitchensis and expressed in Escherichia coli

Fig. 6

GC-MS analysis of the enzymatic product obtained with recombinant P5ßR of P. sitchensis after a 12-h reaction time. The total ion chromatogram was generated from a sample aliquot previously studied by TLC (Fig. 5b, lane 4) and indicates 5ß-pregnane-3,20-dione (3; R t = 21.1 min) originating from progesterone (2; R t = 15.5 min). The 5α-pregnane-3,20-dione (1; R t = 13.8 min) was added to the active reaction as an internal control. It is not produced by enzymatic catalysis as shown in Fig. 5. Peak identification was possible because the substances were tested alone (not shown). Minor peaks do not represent pregnane-like compounds on the basis of a MS data comparison

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