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New Zealand Journal of Forestry Science
Fig. 5 | New Zealand Journal of Forestry Science

Fig. 5

From: The occurrence of progesterone 5β-reductase is not limited to the angiosperms: a functional gene was identified in Picea sitchensis and expressed in Escherichia coli

Fig. 5

Analysis of different P5ßR enzyme activities by TLC following a 12-h reaction time. a 5β-Pregnane-3,20-dione band (R f = 0.65) obtained from progesterone (R f = 0.53) by the recombinant protein of P. sitchensis (lane 1) compared to negative controls containing heat-inactivated spruce enzyme plus progesterone (lane 2) as well as a progesterone sample without enzyme (lane 3). b Identification of the reaction product, 5β-pregnane-3,20-dione (R f = 0.65), from progesterone (R f = 0.53) after testing recombinant protein of A. thaliana (lane 1), Erysimum crepidifolium (lane 2), and P. sitchensis (lane 3: heat-inactivated enzyme sample; lane 4: reference 5α-pregnane-3,20-dione, R f = 0.40, was added to the active enzymatic reaction as an internal control)

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