Table 1 List of primers and their nucleotide sequences, annealing temperature, number of observed bands and the percentage of polymorphism produced by different ISSR and RAPD markers
Molecular type | Primer code | Sequence 5′-3′ | Annealing Temperature (°C) | No. observed bands | Polymorphism % |
|---|---|---|---|---|---|
ISSR | ISSR 7 | CACACACACACAGT | 53 | 9 | 78 ± 5 |
ISSR 3 | CTCTCTCTCTCTCTCTTG | 53 | 14 | 61 ± 12 | |
ISSR 19 | AGAGAGAGAGAGAGGT | 52 | 9 | 46 ± 27 | |
P 26 | CCACTCTCTCTCTCTCTCT | 51 | 9 | 42 ± 24 | |
P 12 | GAGAGAGAGAGAGAGAT | 52 | 8 | 4 | |
P 5 | ACACACACACACACACG | 52 | 8 | 2 | |
RAPD | OPJ 13 | CCACACTACC | 40 | 8 | 81 ± 4 |
OPO 10 | TCAGAGCGCC | 40 | 10 | 78 ± 9 | |
OPJ 19 | GGACACCACT | 40 | 9 | 75 ± 7 | |
OPA 04 | AATCGGGCTG | 40 | 10 | 70 ± 8 | |
OPJ 04 | CCGAACACGG | 40 | 12 | 65 ± 7 | |
OPA 06 | GGTCCCTGAC | 40 | 8 | 2 | |
OPA 07 | GAAACGGGTG | 40 | 7 | 2 | |
OPJ 14 | CACCCGGATG | 40 | 8 | 2 | |
OPJ 18 | TGGTCGCAGA | 40 | 8 | 2 | |
OPO 11 | GACAGGAGGT | 40 | 8 | 2 | |
OPO 09 | TCCCACGCAA | 40 | 7 | 1 | |
OPA 03 | AGTCAGCCAC | 40 | 7 | 1 | |
OPA 05 | AGGGGTCTTG | 40 | 8 | 1 |