Fig. 6

Sapwood contained chloroplasts. Concurrent confocal fluorescence (left) and transmitted light (right) images of sapwood sections treated without (a) and with methanol and b from a 2-year-old E. bosistoana tree. Ray parenchyma cells in sections that lacked the methanol treatment contained chloroplasts that fluoresced red using 488 nm excitation (arrows in a) but these organelles were not visible following methanol washes which indicated that the chlorophyll had been washed out (b). Scale bar 50 μm. Fluorescence emission spectra using 488 nm excitation from sapwood ray parenchyma treated without and with methanol (c)