Table 3 PCR details of 30 clumps of the three growth forms/sampling locations of D. strictus
From: Genetic variability in different growth forms of Dendrocalamus strictus: Deogun revisited
Name of primer | Sequence of the primer | GC (%) | Total number of amplified bands | No. of monomorphic bands | No. of polymorphic bands | Percent of polymorphic bands | Total no. of loci | Size range (kb) | PIC | Resolving Power |
|---|---|---|---|---|---|---|---|---|---|---|
OPA 11 | 5′-CAATCGCCGT-3′ | 60 | 121 | 2 | 8 | 80.0 | 10 | 0.8–3.0 | 0.27 | 3.8 |
OPA 19 | 5′-CAAACGTCGG-3′ | 60 | 81 | 0 | 13 | 100 | 13 | 0.7–3.0 | 0.28 | 5.4 |
OPA 20 | 5′-GTTGCGATCC-3′ | 60 | 68 | 0 | 7 | 100 | 7 | 0.8–3.0 | 0.33 | 3.5 |
OPN 11 | 5′-TCGCCGCAAA-3′ | 60 | 134 | 0 | 15 | 100 | 15 | 0.4–2.0 | 0.37 | 7.5 |
OPN 13 | 5′-AGCGTCACTC-3′ | 60 | 193 | 2 | 15 | 88.2 | 17 | 0.2–3.0 | 0.30 | 7.7 |
OPN 20 | 5′-GGTGCTCCGT-3′ | 70 | 151 | 0 | 14 | 100 | 14 | 0.2–3.0 | 0.27 | 5.1 |
OPX 12 | 5′-TCGCCAGCCA-3′ | 60 | 110 | 0 | 7 | 100 | 7 | 0.2–1.0 | 0.34 | 3.6 |
Total | 430 | 858 | 4 | 79 | 668.2 | 83 | – | 2.2 | 36.6 | |
Mean | 61.4 | 122.6 | 0.6 | 11.3 | 95.5 | 11.9 | 0.5–2.6 | 0.31 | 5.2 | |