Table 1 Microsatellite regions sequences used in DNA amplification of Nothofagus alpina
From: Mating system and gene flow of Nothofagus alpina (Poepp. & Endl.) Oerst. in a clonal seed orchard
Primer code | Forward primer sequence | Reverse primer sequence | Fluorophore colour | Size (bp) |
|---|---|---|---|---|
P3’ | 5’TGC GCA TAA ACA ACA AAC CAC 3’ | Hex + 5’GTG TCG CTG TGC TAG CTT C 3’ | Green | 164–186 |
P5 | Tet + 5’CCT GCG TCC TAG TTT GAG AAG 3’ | 5’GCA CTG AGA GCA CTT GTT GTG 3’ | Green | 110–124 |
P12 | 5’GCC GCA TGA TCC CAA TAA AAT G 3’ | Tet + AGG GAG CGC CTA TCC TTA AC 3’ | Green | 134–150 |
P23 | 5’AAG AGG CTG CCC TCA ACT C 3’ | Fam + 5’ACA GCC TTC CCT AAC TCA GC 3’ | Blue | 156–180 |
P33 | Fam + 5’GCA GGG TTG ACT GTT GCT TC 3’ | 5’AAC AGC CAT CTT TTA CCT TGC 3’ | Blue | 228–250 |
P34 | 5’GAG AGA CTC ACC GGG TTT GG 3’ | Fam + 5’GCA GCC ATT CAC TGA TTC ACC 3’ | Blue | 124–144 |