Table 1 Specific primers used in PCR analysis to obtain AmSesTPS1 and AmGuaiS1 cDNAs and the primers used in qRT-PCR
Amplification/gene | Primer name | Sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|---|
Internal | SesTPS1 F | 5′-GCACTTGATTTCACCACGAAGCAC-3′ | 930 |
SesTPS1 R | 5′-GCTTCCCACTTCGTTGCCCCGTA-3′ | ||
GuaiS1 F | 5′-ACACCGCACCGCCGAAACG-3′ | 1350 | |
GuaiS1 R | 5′-AGCCGCCGAGATGCCAATTACC-3 | ||
5′ RACE | AmSesTPS1 out | 5′- CTCTCTCAGCTCGTTTTGGTGCAAC-3′ |  |
AmSesTPS1 in | 5′-ACAGTACTTAGCATCCAGCCTTGGG-3′ | ||
AmGuaiS1 out | 5′-GAAGCAGTTGAGAGGTGGGACATTG-3′ | ||
AmGuaiS1 in | 5′-ATGTTGATTGGAAGGGCAGAGTTTG-3′ | ||
3′ RACE | AmSesTPS1 out | 5′-GAATCCACACCAGCAGTTGCCCT-3′ | |
AmGuaiS1 out | 5′-TCCCACGCCTAGACGAATGATTTTG-3′ | ||
ORF | AmSesTPS1 full F | 5′-GCAGCAATCAGGTTATTCAAAGGTC-3′ | 1659 |
AmSesTPS1 full R | 5′-GATTAATCGAGAGGAAGCTGGTGAAC-3′ | ||
AmGuaiS1 full F | 5′-ATGTCTTCGGCAAAACTAGGTTCTGC-3′ | 1644 | |
AmGuaiS1 full R | 5′-TCAGATTTCGATAGCATGACGCAAC-3′ | ||
qRT-PCR/AmSesTPS1 a | SESQ-F | 5′-GCA ACG AAG CGG GAA GCT GTG AA-3′ | 238 |
SESQ-R | 5′-TACCCTAAACGAATGGAAATATAGCG-3′ | ||
qRT-PCR/AmGuaiS1 a | GUAI-F | 5′-TCA GAA GAA GAG GCA TGC AAG AAG A-3′ | 251 |
GUAI-R | 5′-GCA AGC TTC TTG GTT CGG TCA GA-3′ | ||
qRT-PCR/AmRPL b | RPL-F | 5′-CCG GTC TTT TGG TAT CAG ACG C-3′ | 251 |
RPL-R | 5′-CCC GAT AAC CAG GAC GTT CAA G-3′ | ||
qRT-PCR/AmTUA b | TUA-F | 5′-GCC AAG TGA CAC AAG CGT AGG T-3′ | 183 |
TUA-R | 5′-TCC TTG CCA GAA ATA AGT TGC TC-3′ |