Fig. 1
From: Detection of asymptomatic fungal microorganisms in Pinus radiata tissue culture material
PCR products generated from nested PCR amplification using primary primers NSL1/NLB4 followed by the nested primers ITS1-F/ITS4, visualised on a 1 % agarose gel. Lane 1: 1 kb ladder, band sizes (bp) are indicated on the left. Lane 2: primary negative control, NSL1/NLB4 negative control re-amplified with nested primers ITS1-F/ITS4. Lane 3–8: adventitious-axillary shoot PCR products, Lane 3: genotype number 19; Lane 4: 16; Lane 5: 13; Lane 6: 11; Lane 7: 15; and Lane 8: 18. Lane 9: secondary negative control, amplified with primers ITS1-F/ITS4 only