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Table 1 Comparison of DNA extraction methods

From: Extraction of high purity genomic DNA from pine for use in a high-throughput Genotyping Platform

Method Wt. of tissue (mg) Homo-genisation conditions Extraction buffer used(vol; temp) Lysis incubation conditions Centrifug-ation conditions RNase A conc. Incubation 2 conditions Extraction conditions DNA pptn conditions Additional purification
S 300 Pestle & mortar under liquid nitrogen 1 mL CTAB buffer; 65°C 60 min; 65°C 18000× g; 20 min 100 μg/ mL 30 min; 37°C (RNase A digestion) (1) 0.2 vol 5 M NaCl + 1 vol CIA1; then 18000× g 20 min 1 vol cold isopropanol; -20°C O/N3; then 18000× g 30 min None
(2) 1 vol CIA1; then 18000× g 20 min
C 150 Geno/ GrinderTM 2000 with sea sand, 2 stainless steel beads 2 mL CTAB buffer; RT2 Same as for Method S 2000× g; 10 min 100 μg/ mL Same as for Method S Same as for Method S 1 vol RT2 isopropanol; RT2 O/N3; then 18000× g 30 min None
CE Same as for Method C         Ethanol/sodium acetate
CQ Same as for Method C         QIAquick® PCR Purification kit
CZ Same as for Method C         Genomic DNA Clean and Concentrator™ kit
CG Same as for Method C         Genomic-tip 20/G kit
F Same as for Method C FastPrep® instrument, tubes with beads supplied in kit Kit supplied 5 min; on ice Same as for Method S None N/A Kit supplied N/A None
D & D+ 50 Same as for Method C Kit supplied N/A Same as for Method C 250 μg/ mL 10 min; -20°C (SDS precipitation) Kit supplied N/A None
N & N96 100 Same as for Method C Kit supplied Same as for Method S Same as for Method C 300 μg/ mL Same as for Method D Kit supplied N/A None
  1. 1 Chloroform:isoamyl alcohol (24:1) vol:vol.
  2. 2 Room temperature.
  3. 3 Overnight.