Molecular tools for differentiating Cyclaneusma minus morphotypes and assessing their distribution in Pinus radiata forests in New Zealand

New Zealand Journal of Forestry Science

Table 1 Primers used in this study

Gene region	Primer set	Sequence (5′–3′)	Annealing temperature (°C)	Reference
ITS	ITS1-F	CCTGGTCATTTAGAGGAAGTAA	55	(Gardes and Bruns 1993)
ITS	ITS4	TCCTCCGCTTATTGATATGC	55	(Gardes and Bruns 1993)
Cm^a ‘simile’ specific ITS	Cm ‘simile’ F	CCGGGCCTTATGGTCCGC	63	(This study)
Cm^a ‘simile’ specific ITS	Cm ‘simile’ R	CAGGCGCCAGCCCAGCG	63	(This study)
Cm^a ‘verum’ specific ITS	Cm ‘verum’ F	CCGGGCCTTCGGGCCTAC	63	(This study)
Cm^a ‘verum’ specific ITS	Cm ‘verum’ R	CAGGCACCAACCCAGGC	63	(This study)

^a Cm Cyclaneusma minus. These primers were used for both conventional and real-time PCR