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Table 3 Microarray probe targets for quantitative RT-PCR validation

From: Heterologous hybridisation to a Pinus microarray: profiling of gene expression in Pinus radiata saplings exposed to ethephon

Key process

Gene

Probe ID

5′ Primer

3′-Primer

PCR validation

Catechin Biosynthesis

Anthocyanidin reductase

Contig38855a

TGGCATCGACGAAATCTACG

TTTCGCGGATTCTACAGCGT

Yes

Isoflavonoid biosynthesis

Isoflavone reductase

Contig32761c

CCCGACGTTGAATACACATCAGT

ACGGCTCAAATAACGCTCGACT

Yes

Mono terpenoids

Pinene synthase

Contig819c

CTAACTTAGGTCTCTGATTT

GATATCTTACCCACAACATAGC

Yes

Unknown

Unknown

Contig24580b

AAATATAATCTCTGTCATCTGCG

AAGCGAATGGTCATGGTC

Yes

Cell Cycle

Cyclophilin

Pta121982552a

AGAGTCGATCTACGGCGAGA

CTTTACAAAGTTCTCATCGGCA

Yes